RNA Preparation and Purification
Zymo Research Corporation Direct-zol™ RNA MiniPrep (200 Preps) w/ Zymo-Spin™ IIC Columns (Capped)
Total RNA including small RNAs (17-200 nt)2, is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, samples stored in DNA/RNA Shield™, etc.).Simply add ethanol to a TRI Reagent sample, bind directly to the Zymo-Spin™ Column, wash, and elute RNA. No phase separation, precipitation, or post-purification steps are necessary. RNA is high-quality and ready for Next-Gen Sequencing, RTqPCR, transcription profiling, hybridization, etc.
Active Motif Low Cell ChIP Kit 16 rxns
Active Motif's Low Cell ChIP-Seq Kit provides a complete ChIP-Seq workflow for chromatin preparation, immunoprecipitation and NGS library preparation from limited amounts of cells or small tissue biopsies. The assay is robust enough to work with both high abundance histone modifications and low abundance transcription factor proteins. The Next Gen DNA Library and Next Gen DNA Indexing reagents are included so you can generate high complexity sequencing libraries that are compatible with Illumina platforms.
HORIZON DISCOVERY BIOSCIENCES LIMITED CRISPRa Lentiviral dCas9-VPR Reagents, hEF1a, Viral Particles - 50µL, 10^7 TU/mL
The CRISPR activation (CRISPRa) system is a variation of the canonical CRISPR-Cas9 used for creation of double-strand breaks in genomic DNA. It utilizes a nuclease-deactivated S. pyogenes Cas9 (dCas9), often called "dead Cas9", that is fused to one or more transcriptional activators. When paired with a well-designed guide RNA that targets a gene near a promoter region, the gene's native transcription start site is activated.
Omega Biotek Inc Mag-Bind Total RNA 96 Kit | 4 x 96 preps
The Mag-Bind Total RNA 96 Kit provides a novel technology for total RNA isolation. This kit allows the rapid and reliable isolation of high-quality total cellular RNA and viral RNA from a wide variety of cells and tissues. Unlike column-based systems, the binding of nucleic acids to magnetic particles occurs in solution resulting in increased binding kinetics and binding efficiency. Particles are also completely re-suspended during the wash steps of the purification protocol, which improves the removal of contaminants and increases nucleic acid purity. Mag-Bind Total RNA 96 Kit procedure can be fully automated with most robotic workstations.
Norgen Biotek Corp RNase-Free DNase I Kit, 200 Reactions
Norgen's RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen's RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen's RNA purification kits. Alternatively, after isolating total RNA using one of Norgen's RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen's RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.
Apexbio Technology LLC HyperScribe™ T7 High Yield RNA Synthesis Kit, 50 rxns
HyperScribe™ T7 High Yield RNA Synthesis Kit performs in vitro RNA transcription experiment in a relatively short time using T7 RNA polymerase. The kit is designed for high yields of RNA transcripts, highly specific radiolabeled RNA probes and to obtain capped, dye-labeled or biotinylated RNA incorporated modified nucleotides. RNA synthesized using this kit covers a wide range of applications, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots. The kit contains sufficient reagents to carry out 25/50/100 reactions, 20 l each time. Up to 180 g of RNA can be generated with 1 g of control template per standard reaction. Each 25/50/100 reactions kit can produce up to 4.5 mg/9 mg/18 mg RNA. Other sizes are also available. Please inqury us for quote.
Sigma Aldrich Fine Chemicals Biosciences RNA ZAP CASE OF 6X 250 ML
RNASE ZAP 6x250ml. A cleaning agent for removing RNase from glassware, plastic surfaces, countertops, and pipettors. It is also effective at eliminating RNase contamination from microcentrifuge tubes without inhibiting subsequent enzymatic reactions.